Scientific advances in assisted reproduction have raised important legal challenges that require in-depth analysis in the context of the Colombian Civil Code. This article examines the main legal issues, including the determination of parentage, the rights of children born through assisted reproductive techniques, and the storage of gametes and embryos. Colombian legislation is compared with that of other countries, highlighting legal gaps and proposing necessary reforms for a more adequate and equitable legal framework and also the pronouncements of Colombian courts in jurisprudence that have helped to glimpse both the challenges and procedural weaknesses in this issue.